Genetics for beginners | Genes Alleles Loci on Chromosomes |
นอกจากการดูบทความนี้แล้ว คุณยังสามารถดูข้อมูลที่เป็นประโยชน์อื่นๆ อีกมากมายที่เราให้ไว้ที่นี่: ดูเพิ่มเติม
This video lecture explains
1. What is genetics?
2. What is a trait?
3. What is an allele?
4. What is the relationship between genes, alleles, DNA and chromosome?
5. What are heterozygous organisms?
6. What are homozygous organisms?
7. What is the difference between genotype and phenotype?
8. What is the difference between homozygous dominant and homozygous recessive?
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Homogeneous vs Heterogeneous | homogenous | by #AgriCareAS |
Welcome to our channel AgricareAS.This video is all about two confusing terminologies Homogeneous and Heterogeneous.Homogeneous plant population is a population in which all the individuals in the population are phenotypically & genetically similar
Whereas,
Heterogeneous population is a population in which population is genotypically dissimilar but phenotypically similar!
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Homozygous vs. Heterozygous Samples in qPCR — Ask TaqMan #23
Submit your question: http://bit.ly/1cgFftk
Did you know that TaqMan assays can also be used for SNP Genotyping? While the assays use the same robust TaqMan chemistry, they contain two probes, and thus analysis is different than when using a Gene Expression assay. This prompted our next question, from Melissa Arria from the IVIC in Venezuela, who wondered was seeing amplification from both probes from a sample she knew was homozygous. She asked us: “How can I differentiate real homozygous samples from heterozygous samples?” Great question! But first, let’s review how the assay works to understand what we are seeing.
Like a Gene Expression assay, A TaqMan Genotyping assay contains Sequencespecific forward and reverse primers to amplify the polymorphic sequence of interest. However, as we mentioned, the SNP assays are unique since they contain not one, but TWO TaqMan® MGB probes.
One probe labeled with VIC® dye detects the Allele 1 sequence
One probe labeled with FAM™ dye detects the Allele 2 sequence
Note that all Genotyping assays use the same notation for the context sequence, which is the immediate sequence around the SNP. The first base in brackets is detected by the VIC probe, and the second base by the FAM probe. You can find all this information online for every assay.
The assays are combined with maste rmix and 120 ng of purified gDNA, and then amplified. Data can be collected in realtime or off line, as analysis is taken from the end point read of the fluorescence.
The sample will either be homozygous for allele 2, which means we will see mostly VIC dye, homozygous for allele 1, which means we will see mostly FAM signal, or a heterozygous, which means there will be about equal contribution of signal from each dye. When viewed across an entire plate of samples, the data will typically resolve into 3 discrete clusters, assuming enough samples were used to display a normal distribution given the minor allele frequency for your SNP. Note that there are times in which you would not expect to see 3 clusters, such as when the gene is on the X chromosome, if a copy number variation is involved, or if the minor allele frequency is extremely low.
Let’s take a closer look at what’s happening in each reaction. For example, this sample is a VIC dye homozygous call. However, if we look at the real time data, we’ll see some amplification of both probes.
Heterozygous and Homozygous l Genetics l BSc Final year Zoology 2nd paper l by Irshad ali
Irshad ali motivation and education presents
Educational video for students it’s a Heterozygous and Homozygous so please watch and share your thoughts with me 🙏🙏
Irshadalimotivationanandeducation
इस वीडियो में हम बात करेंगे Heterozygous and Homozygous की वो भी बिल्कुल आसान भाषा में और ये Bsc Final year वालों के लिए important topic है
So if you like this video please share with your friends and finally subscribe the channel
Definition of Heterozygous – Genetic Basis of Inheritance – Biology Class 12
Video Lecture on Definition of Heterozygous from Genetic Basis of Inheritance chapter of Class 12 Biology for HSC, CBSE & NEET.
Watch Previous Videos of Chapter Genetic Basis of Inheritance:
1) Definition of Gene Symbols Genetic Basis of Inheritance Biology Class 12 https://www.youtube.com/watch?v=6WDSYzFhsI
2) Definition of Homozygous Genetic Basis of Inheritance Biology Class 12 https://www.youtube.com/watch?v=AdzQID0Oy0U
Watch Next Videos of Chapter Genetic Basis of Inheritance:
1) Test Cross in Genetics Genetic Basis of Inheritance Biology Class 12 https://www.youtube.com/watch?v=SIrh25FljE
2) Polygenic Inheritance Genetic Basis of Inheritance Biology Class 12 https://www.youtube.com/watch?v=gEJdK3Sjxg
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